Translational Surgery

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 4  |  Issue : 2  |  Page : 17--21

Pro-inflammatory chemokine interleukin-17A may increase venous thrombus resolution by increasing venous thrombus neovascularization


Wang Ruihua, Wu Xiaoyu, Li Bo, Lu Xinwu 
 Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine; Vascular Center of Shanghai Jiaotong University, Shanghai, China

Correspondence Address:
Lu Xinwu
Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Vascular Center of Shanghai Jiaotong University, 639 Zhizaoju Road, Shanghai 200011
China

Objectives: The aim of this study was to assess the effect of the pro-inflammatory interleukin (IL)-17A in deep venous thrombosis (DVT) resolution. Materials and Methods: A total of 45 DVT patients, 30 primary deep venous insufficiencies (DVI) patients, and 18 healthy volunteers were divided into three groups: (a) Control group, (b) DVT group, and (c) DVI group. The venous blood samples of the lower extremity with DVT were collected to evaluate the expression of IL-17A in plasma. Samples of superficial venous thrombus and superficial vein without thrombosis were collected to evaluate the expression of IL-17A in tissue. Male C57/BL mice DVT model was established and was randomly divided into five groups: (1) Control group, no treatment or surgical intervention; (2) Sham group, no treatment and sham operation; (3) DVT group, each mouse intravenous (iv) injected with phosphate-buffered saline (PBS); (4) IL-17A group, each mouse was iv injected with IL-17A; and (5) IL-17A-neutralizing antibody (NA) group, each mouse was iv injected with IL17A NA. Inferior vena cava (IVC) with thrombi were collected to measure their length and weight and analysis CD31(+) endothelial cells in thrombus of internal cerebral vein. Results: Western blot suggested that the expression of IL-17A in superficial vein with thrombosis was higher than superficial vein without thrombosis from human samples (P < 0.05). Comparing the plasma IL-17A levels in human samples, it was found that the expression of IL-17A in DVT and DVI groups was higher than those control group (P < 0.05). In C57/BL mice DVT model, immunofluorescence showed that CD31(+) endothelial cells in thrombus in IL-17A intervention group were more than those in other groups (P < 0.05). The weight of IVC with thrombi in IL-17A intervention group were less than those in other groups (P < 0.05), however, without statistical difference compared with the DVT group and IL-17A-NA group. Conclusions: Venous thrombus neovascularization can be increased by pro-inflammatory chemokine IL-17A but do not appear to decrease thrombus size.


How to cite this article:
Ruihua W, Xiaoyu W, Bo L, Xinwu L. Pro-inflammatory chemokine interleukin-17A may increase venous thrombus resolution by increasing venous thrombus neovascularization.Transl Surg 2019;4:17-21


How to cite this URL:
Ruihua W, Xiaoyu W, Bo L, Xinwu L. Pro-inflammatory chemokine interleukin-17A may increase venous thrombus resolution by increasing venous thrombus neovascularization. Transl Surg [serial online] 2019 [cited 2020 Jun 4 ];4:17-21
Available from: http://www.translsurg.com/article.asp?issn=2468-5585;year=2019;volume=4;issue=2;spage=17;epage=21;aulast=Ruihua;type=0